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human sox9 antibody  (Bio-Techne corporation)


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    Bio-Techne corporation human sox9 antibody
    Human Sox9 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 378 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+sox9+antibody/Human+SOX9+Antibody/custom%40af3075%4042531129
    Average 96 stars, based on 378 article reviews
    human sox9 antibody - by Bioz Stars, 2026-08
    96/100 stars

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    Production and identification of human gallbladder epithelial cells (hGBECs) (A) Schematic diagram for the procedure of production and identification of hGBECs. (B) q-RT-PCR for expression of KRT19, EPCAM, LGR5, <t>SOX9,</t> HNF4A and ALB in each passage of hGBECs. Fold chang is calculated by 2 -ΔΔCt , relative to GAPDH (n= 3). Data are represented as mean ± SD. (C) Immunostaining for HNF4A, SOX9 (red), TROP2 and EPCAM (green) of hGBECs. Cell nuclei were stained by DAPI (blue). Scale bar = 100 μm.
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    Production and identification of human gallbladder epithelial cells (hGBECs) (A) Schematic diagram for the procedure of production and identification of hGBECs. (B) q-RT-PCR for expression of KRT19, EPCAM, LGR5, <t>SOX9,</t> HNF4A and ALB in each passage of hGBECs. Fold chang is calculated by 2 -ΔΔCt , relative to GAPDH (n= 3). Data are represented as mean ± SD. (C) Immunostaining for HNF4A, SOX9 (red), TROP2 and EPCAM (green) of hGBECs. Cell nuclei were stained by DAPI (blue). Scale bar = 100 μm.
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    Production and identification of human gallbladder epithelial cells (hGBECs) (A) Schematic diagram for the procedure of production and identification of hGBECs. (B) q-RT-PCR for expression of KRT19, EPCAM, LGR5, <t>SOX9,</t> HNF4A and ALB in each passage of hGBECs. Fold chang is calculated by 2 -ΔΔCt , relative to GAPDH (n= 3). Data are represented as mean ± SD. (C) Immunostaining for HNF4A, SOX9 (red), TROP2 and EPCAM (green) of hGBECs. Cell nuclei were stained by DAPI (blue). Scale bar = 100 μm.
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    Production and identification of human gallbladder epithelial cells (hGBECs) (A) Schematic diagram for the procedure of production and identification of hGBECs. (B) q-RT-PCR for expression of KRT19, EPCAM, LGR5, SOX9, HNF4A and ALB in each passage of hGBECs. Fold chang is calculated by 2 -ΔΔCt , relative to GAPDH (n= 3). Data are represented as mean ± SD. (C) Immunostaining for HNF4A, SOX9 (red), TROP2 and EPCAM (green) of hGBECs. Cell nuclei were stained by DAPI (blue). Scale bar = 100 μm.

    Journal: STAR Protocols

    Article Title: Protocol for generation of human gallbladder epithelia cells and their derived hepatocytes using a chemically defined approach

    doi: 10.1016/j.xpro.2025.104302

    Figure Lengend Snippet: Production and identification of human gallbladder epithelial cells (hGBECs) (A) Schematic diagram for the procedure of production and identification of hGBECs. (B) q-RT-PCR for expression of KRT19, EPCAM, LGR5, SOX9, HNF4A and ALB in each passage of hGBECs. Fold chang is calculated by 2 -ΔΔCt , relative to GAPDH (n= 3). Data are represented as mean ± SD. (C) Immunostaining for HNF4A, SOX9 (red), TROP2 and EPCAM (green) of hGBECs. Cell nuclei were stained by DAPI (blue). Scale bar = 100 μm.

    Article Snippet: Anti-human SOX9 rabbit primary antibody (1:200 IF) , Merck , Cat#HPA001758; RRID: AB_1080067.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Immunostaining, Staining

    Quality tests of hGBECs (Passage 3) (A) Growth curves of hGBECs at different densities (n=3). Data are represented as mean ± SD. (B) Table displaying sterility test results of hGBECs. (C) Flow cytometric analysis showing the proportion of SOX9+, TROP2+, HNF4A+, EPCAM+ cells. (D) Representative karyotype of Passage 3 cells.

    Journal: STAR Protocols

    Article Title: Protocol for generation of human gallbladder epithelia cells and their derived hepatocytes using a chemically defined approach

    doi: 10.1016/j.xpro.2025.104302

    Figure Lengend Snippet: Quality tests of hGBECs (Passage 3) (A) Growth curves of hGBECs at different densities (n=3). Data are represented as mean ± SD. (B) Table displaying sterility test results of hGBECs. (C) Flow cytometric analysis showing the proportion of SOX9+, TROP2+, HNF4A+, EPCAM+ cells. (D) Representative karyotype of Passage 3 cells.

    Article Snippet: Anti-human SOX9 rabbit primary antibody (1:200 IF) , Merck , Cat#HPA001758; RRID: AB_1080067.

    Techniques: Sterility